Neu-Millennia Scientific
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$99.00 per ml

  • Replaces hazardous ethidium bromide (EtBr)

  • Better sensitivity than EtBr (down to 0.1ng)

  • Excitation of both UV and blue light

  • Ships at ambient temperature (store at 4°C)

  • Two types: PS (Pre-Stain) or LD (Loading Dye)

  • Compatible with SmartBlue™ Transilluminator

  • Dispose per your facility's SOP for non-hazardous waste

  • 1/2 the price of EtBr

 

Ordering Information:

E4500-PS   SmartGIowTM Pre Stain 20,000 x concentration  $  99.00 1.0ml

E4500-LD   SmartGIowTM Loading Dye with Stain (6x)*        $  99.00 1.0ml

E4000         SmartBIueTM Blue Light transilluminator             $549.00 each

                *Storage; 40C for 2 years


Dispose per your facility's SOP for non-hazardous waste

 
 

SmartGlow TMSafe Green Stain FAQs

 

Q: What is the difference between SmartGlowTM PS and SmartGlowTM LD?

A: PS (Pre Stain) is used like EtBr, a small amount is added to the agarose solution before pouring the gel. LD (Loading Dye) is added to the sample prior to pipetting into the gel wells. Both types of SmartGlowTM are considered safer than EtBR. They are non-hazardous for disposal and are excited using blue light or UV light

 

Q: Does SmartGlowTM LD Loading Dye slow down or affect the separation of molecules (vs. non-stained nucleic acid samples)?
A: It is possible for the bound dye to slightly slow speed of migration, but generally not enough to significantly affect results. The SmartGlowTM PS is added to the agarose prior to electrophoresis and will have less effect on migration rate.

 

 
Q: Can the SmartGlowTM PS be used in a post stain process instead of pre-staining the gel?
A: The Pre Stain is not designed for post-staining gels.

 

 
Q: When is the SmartGlowTM PS (Pre Stain) added to Agarose?

A: Add the appropriate amount of SmartGlowTM PS to the agarose (5ul per 100ml solution) after the microwaving or heating step. It is not recommended to add the stain before microwaving.

 
Q: What are the shipping and storage conditions recommended for the SmartGlowTM Stains?
A: SmartGlowTM stains should be stored in their opaque tubes at 40C and at this storage temperature they have a shelf life of 2+ years. The SmartGlowTM stains should not be frozen. The stains are shipped at ambient temperature and are stable for up to 7 days outside of cold storage.
 
 
Q: What are the excitation and emission wavelengths for SmartGlowTM Stains?

A: Both SmartGlowTM PS and LD have excitation peaks at 290nm (UV) and 490 nm (blue), and emission peaks at 520nm and 635nm.

 

Q: Are SmartGlowTM stains hazardous?

A: SmartGlowTMproducts are considered safer than Ethidium Bromide. They are non-carcinogenic as determined by the Ames-test, with negative results in both mouse marrow chromophilous etfflhrocyte micronucleus and mouse primary spermatocyte chromosomal aberration tests. However, all laboratory chemicals and reagents should be handled with caution and you should wear gloves and avoid skin contact.

Q: What solvents are used in the SmartGlowTM reagents?
A: SmartGlowTM PS is supplied in water; SmartGlowTM LD is supplied in 50% DMSO.

 

Q: How can SmartGlowTM Stains be disposed of?

A: SmartGlowTMstains are considered non-hazardous waste as they are non-carcinogenic, do not contain heavy metals, are non-corrosive, non-flammable and non-reactive. They can be safely disposed down the drain or per your facility's SOP for non-hazardous waste.

 

 

Q: What is the sensitivity of SmartGlowTM stains:

A: SmartGlowTMPS has a sensitivity range for visualization of 0.1-0.3ng of nucleic acid per band. SmartGlowTM LD has a sensitivity range of 0.2-0.6ng of nucleic acid per band.

 

Q: Is there a difference in excitation level using UV vs. blue light for SmartGlowTM?

A: UV light provides for slightly higher emission signal for SmartGlowTM LD and PS.

 

Q: What is the loading dye included in the SmartGlowTM LD?

A: Yes, SmartGlowTM LD is supplied with the tracking dye bromophenol blue in a concentration.

 

Q: After running a gel using SmartGlowTM PS, is there a recommended procedure for destaining?

A: Destaining should not be required. If there is significant background fluorescence, eliminate or decrease the amount of PS added to the running buffer.